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Acupuncture Research  针灸研究

Influence of Acupuncture on Microcirculation Perfusion of Pericardium Meridian and Heart in Acute Myocardial Ischemia Model Rats*
针刺对急性心肌缺血模型大鼠心包经和心脏微循环灌注的影响*

ZHUANG Yi 1 , 2 1 , 2 ^(1,2){ }^{1,2}, ZHOU Jie 1 , 3 1 , 3 ^(1,3){ }^{1,3}, ZHOU Yu-mei 1 , 4 1 , 4 ^(1,4){ }^{1,4}, CHEN Jiao 1 1 ^(1){ }^{1}, WU Ping 1 1 ^(1){ }^{1}, LYU Pei-ran 1 1 ^(1){ }^{1}, WAN Min 1 1 ^(1){ }^{1}, LUO Liao-jun 1 1 ^(1){ }^{1}, CAI Ding-jun 1 1 ^(1){ }^{1}, and LIANG Fan-rong 1 1 ^(1){ }^{1}
庄毅 1 , 2 1 , 2 ^(1,2){ }^{1,2} ,周杰 1 , 3 1 , 3 ^(1,3){ }^{1,3} ,周玉梅 1 , 4 1 , 4 ^(1,4){ }^{1,4} ,陈娇 1 1 ^(1){ }^{1} ,吴平 1 1 ^(1){ }^{1} ,吕培然 1 1 ^(1){ }^{1} ,万敏 1 1 ^(1){ }^{1} ,罗廖军 1 1 ^(1){ }^{1} ,蔡定军 1 1 ^(1){ }^{1} ,梁帆荣 1 1 ^(1){ }^{1}

Abstract  摘要

Objective: To observe the influence of acupuncture on microcirculation perfusion of the pericardium meridian and heart in acute myocardial ischemia (AMI) rats and evaluate whether acupuncture can simultaneously affect the meridians and corresponding viscera. Additionally, acupoints at different meridians were compared and whether they exert the same effects was discussed. Methods: Totally 32 Sprague-Dawley rats were subjected to left anterior descending (LAD) ligation to develop an AMI model. Rats were divided into 4 groups, including AMI, acupuncture Neiguan (PC 6), Lieque (LU 7) and Qiansanli (LI 10) groups ( n = 8 n = 8 n=8n=8 ). Eight rats received only thoracotomy (sham-operated group). The rats in the acupuncture groups received manual acupuncture at PC 6, LU 7 and LI 10 acupoints for 15 min, respectively. The microcirculation perfusion of pericardium meridian and heart was monitored by laser speckle perfusion imager (LSPI) before, during and after acupuncture manipulation for 15 min . Subsequently, the perfusion unit (PU) was calculated and analyzed by PSI System. Results: After LAD, compared to pre-acupuncture stage, the heart microcirculation perfusion (HMP) in the AMI group decreased continuously at during-acupuncture ( P > 0.05 P > 0.05 P > 0.05P>0.05 ) and post-acupuncture stages ( P < 0.05 P < 0.05 P < 0.05P<0.05 ), and the pericardium meridian microcirculation perfusion (PMP) showed no significant differences at 3 stages ( P > 0.05 P > 0.05 P > 0.05P>0.05 ). Compared to pre-acupuncture stage, the PMP and HMP in PC 6 group significantly increased during acupuncture manipulation (both P < 0.05 P < 0.05 P < 0.05P<0.05 ), and PMP decreased obviously after acupuncture ( P < 0.05 P < 0.05 P < 0.05P<0.05 ). The PMP in the LU 7 and LI 10 groups were slightly elevated (both P > 0.05 P > 0.05 P > 0.05P>0.05 ); however, they were significantly reduced after acupuncture manipulation (both P < 0.05 P < 0.05 P < 0.05P<0.05 ). Additionally, HMP of LI 10 group was decreased significantly during acupuncture, especially compared to pre-acupuncture stage ( P < 0.05 P < 0.05 P < 0.05P<0.05 ). Conclusions: Acupuncture at PC 6 obviously increased the PMP and HMP in AMI rats, and the effects were superior to at LU 7 and LI 10 acupoints. It was further confirmed that acupuncture promoted qi and blood circulation, indicating that acupoint specificity exists and features a meridian-propagated effect.
目的:观察针刺对急性心肌缺血(AMI)大鼠心包经和心脏微循环灌注的影响,并评估针刺是否能同时影响经脉与相应脏腑。此外,比较不同经脉的穴位,探讨其是否产生相同效果。方法:共 32 只斯普林格-达利耶大鼠行左前降支(LAD)结扎建立 AMI 模型。大鼠分为 4 组,包括 AMI 组、针刺内关(PC 6)、列缺(LU 7)和足三里(LI 10)组( n = 8 n = 8 n=8n=8 )。8 只大鼠仅接受开胸手术(假手术组)。针刺组分别在内关、列缺和足三里穴位进行手动针刺 15 分钟。通过激光散斑灌注成像仪(LSPI)在针刺操作前、中、后 15 分钟监测心包经和心脏的微循环灌注。随后,通过 PSI 系统计算并分析灌注单位(PU)。 结果:在 LAD 后,与针刺前阶段相比,AMI 组的 心脏微循环灌注(HMP)在针刺时( P > 0.05 P > 0.05 P > 0.05P>0.05 )和针刺后阶段( P < 0.05 P < 0.05 P < 0.05P<0.05 ) 持续下降,而心包经微循环灌注(PMP)在三个阶段( P > 0.05 P > 0.05 P > 0.05P>0.05 )均无显著差异。与针刺前阶段相比,PC 6 组的 PMP 和 HMP 在针刺操作期间(均 P < 0.05 P < 0.05 P < 0.05P<0.05 )显著增加,而针刺后 PMP 明显下降( P < 0.05 P < 0.05 P < 0.05P<0.05 )。LU 7 组和 LI 10 组的 PMP 略有升高(均 P > 0.05 P > 0.05 P > 0.05P>0.05 );然而,针刺操作后 PMP 显著降低(均 P < 0.05 P < 0.05 P < 0.05P<0.05 )。此外,LI 10 组的 HMP 在针刺期间显著下降,尤其与针刺前阶段相比( P < 0.05 P < 0.05 P < 0.05P<0.05 )。结论:针刺 PC 6 能明显增加 AMI 大鼠的 PMP 和 HMP,其效果优于 LU 7 和 LI 10 穴位。进一步证实了针刺能促进气血循环,表明穴位具有特异性,并呈现经气传导效应。

KEYWORDS microcirculation perfusion, acupuncture, pericardium meridian, heart, acute myocardial ischemia
关键词 微循环 血流灌注,针灸,心包经,心脏,急性心肌缺血
The meridian system is an essential pathway system in Chinese medicine (CM), serving as the core theory behind various therapies, such as acupuncture, massage, moxibustion, and qigong. ( 1 , 2 ) ( 1 , 2 ) ^((1,2)){ }^{(1,2)} The meridian is an important pathway of contact and communication between the internal organs and outside limbs and joints of the human body. The meridian acupoints are located on body surface along the pathways between 12 main meridians and 2 extra meridians. Stimulation of the acupoints can promote benign adjustment effects on organ functions and related meridians.
经络系统是中医(CM)中一个重要的通路系统,是针灸、按摩、艾灸和气功等多种疗法背后的核心理论。经络是人体内脏与四肢关节之间接触和沟通的重要通路。经络穴位位于人体表面,沿着 12 正经和 2 个奇经的通路分布。刺激穴位可以促进器官功能和相关经络的良好调节作用。
For decades, scientists have been trying to determine the biological characteristics of the relationships between meridians and viscera.
几十年来,科学家们一直在努力确定经络与脏腑之间关系的生物学特征。
Some studies have found that the concentrations of some substances and factors, such as adenosine
一些研究发现,某些物质和因素的浓度,如腺苷
triphosphate and adenosine (ADO), ( 3 , 4 ) ( 3 , 4 ) ^((3,4)){ }^{(3,4)} oxygen partial pressure, ( 5 ) ( 5 ) ^((5)){ }^{(5)} electrical skin impedance, ( 6 ) ( 6 ) ^((6)){ }^{(6)} transcutaneous CO 2 CO 2 CO_(2)\mathrm{CO}_{2} emission, ( 7 ) ( 7 ) ^((7)){ }^{(7)} and temperature at the acupoints (meridians) were higher than those at the non-acupoints (non-meridians). Additionally, microcirculation perfusion has been accepted in the field of meridian research, which might reflect the qi and blood of meridians, as well as their functions. ( 8 12 ) ( 8 12 ) ^((8-12)){ }^{(8-12)} These studies mostly adopted laser doppler flowmeter (LDF), diffuse reflectance spectroscopy, ( 13 ) ( 13 ) ^((13)){ }^{(13)} orthogonal polarization spectral imaging, ( 14 ) ( 14 ) ^((14)){ }^{(14)} and laser speckle contrast imaging. ( 15 ) ( 15 ) ^((15)){ }^{(15)} Among them, the laser speckle perfusion image (LSPI) is an emerging perfusion imaging technology that combines the advantages of high image density and speed, thus enabling the accurate capture of dynamic microcirculation changes. ( 16 ) ( 16 ) ^((16)){ }^{(16)} This technique achieves good results in the detection of perfusion in organs and tissues with rich microcirculation systems ( 17 19 ) ( 17 19 ) ^((17-19)){ }^{(17-19)} and dermal areas around acupoints. ( 20 ) ( 20 ) ^((20)){ }^{(20)} However, most previous studies focused on the distinction between the acupoints (meridians) and non-acupoints (non-meridians). The simultaneous changes of meridian and the corresponding viscera, especially stimulation acupoints at different meridians, remain unclear and relatively rare.
三磷酸腺苷和腺苷(ADO), ( 3 , 4 ) ( 3 , 4 ) ^((3,4)){ }^{(3,4)} 氧分压, ( 5 ) ( 5 ) ^((5)){ }^{(5)} 皮肤电阻抗, ( 6 ) ( 6 ) ^((6)){ }^{(6)} 经皮 CO 2 CO 2 CO_(2)\mathrm{CO}_{2} 透射, ( 7 ) ( 7 ) ^((7)){ }^{(7)} 以及穴位(经络)的温度均高于非穴位(非经络)。此外,微循环灌注已被经络研究领域接受,它可能反映经络的气血及其功能。 ( 8 12 ) ( 8 12 ) ^((8-12)){ }^{(8-12)} 这些研究大多采用激光多普勒血流计(LDF)、漫反射光谱、 ( 13 ) ( 13 ) ^((13)){ }^{(13)} 正交偏振光谱成像、 ( 14 ) ( 14 ) ^((14)){ }^{(14)} 和激光散斑对比成像。 ( 15 ) ( 15 ) ^((15)){ }^{(15)} 其中,激光散斑灌注成像(LSPI)是一种新兴的灌注成像技术,它结合了高图像密度和速度的优点,从而能够准确捕捉动态微循环变化。 ( 16 ) ( 16 ) ^((16)){ }^{(16)} 该技术在检测具有丰富微循环系统的器官和组织以及穴位周围的皮肤区域方面取得了良好效果。 ( 17 19 ) ( 17 19 ) ^((17-19)){ }^{(17-19)} 然而,大多数先前研究集中于穴位(经络)与非穴位(非经络)之间的区别。 经络与相应脏腑的同步变化,尤其是不同经络上刺激穴位的情况,仍不明确且相对少见。
Based on previous studies, our present study was carried out with LSPI technology and utilized an acute myocardial ischemia (AMI) rat model to observe whether microcirculation perfusion could change in the pericardium meridian and heart during acupuncture stimulation Neiguan (PC 6). This differences in acupuncture stimulation of PC 6, Lieque (LU 7) and Qiansanli (LI 10) at different meridians were further compared.
基于既往研究,本研究采用激光散斑成像(LSPI)技术,利用急性心肌缺血(AMI)大鼠模型,观察针刺刺激内关(PC 6)时,心包经和心脏的微循环灌注是否发生变化。进一步比较了针刺不同经络的内关(PC 6)、列缺(LU 7)和三阴交(LI 10)的差异。

METHODS  方法

Animals and Grouping  动物与分组

Specific pathogen free (SPF) Sprague-Dawley rats ( 1.8 ± 2 1.8 ± 2 1.8+-21.8 \pm 2 months, weighing 250 ± 50 g 250 ± 50 g 250+-50g250 \pm 50 \mathrm{~g} ) with an equal male to female ratio were obtained from the Experimental Animal Center of Sichuan Province People’s Hospital [Certificate No. SCXK (Chuan) 2013-15]. These rats were supplied with ad libitunm acess to standard rat chow and water, and housed in a reversed 12 h 12 h 12-h12-\mathrm{h} light/dark condition. The temperature remained at 21 23 C 21 23 C 21-23^(@)C21-23^{\circ} \mathrm{C} and a relative humidity of 40 % 70 % 40 % 70 % 40%-70%40 \%-70 \%. Four rats were kept in a dry-raised rat cage without any animal bedding, and males and females were separated. All experiments in this study
从四川省人民医院实验动物中心获取无特定病原体(SPF)级斯普林格-达利耶大鼠( 1.8 ± 2 1.8 ± 2 1.8+-21.8 \pm 2 月龄,体重 250 ± 50 g 250 ± 50 g 250+-50g250 \pm 50 \mathrm{~g} ),雌雄比例相等。[证书编号 SCXK(川)2013-15]。这些大鼠可自由获取标准大鼠饲料和水,饲养在逆转的 12 h 12 h 12-h12-\mathrm{h} 光/暗条件下。温度维持在 21 23 C 21 23 C 21-23^(@)C21-23^{\circ} \mathrm{C} ,相对湿度为 40 % 70 % 40 % 70 % 40%-70%40 \%-70 \% 。四只大鼠被饲养在无任何动物垫料的干燥饲养笼中,雌雄分开。本研究所有实验

were performed in accordance with national laws and guidelines and were approved by the Laboratory Animal Ethics of Chengdu University of Traditional Chinese Medicine. All procedures were conducted and completed at the Key Laboratory of AcupunctureMoxibustion in Chengdu University of Traditional Chinese Medicine.
均依照国家法律和指南进行,并获得成都中医药大学实验动物伦理委员会批准。所有程序均在成都中医药大学针灸学重点实验室完成。
A total of 40 rats were randomly divided into 3 groups according to sex and weight, including shamoperated, AMI, and acupuncture groups with a ratio of 1:1:3. In addition, the rats in the acupuncture group were further randomly assigned to 3 subgroups, including PC 6, LU 7 and LI 10 groups, 8 rats in each group.
共 40 只大鼠按性别和体重随机分为 3 组,包括假手术组、心肌梗死组和针刺组,比例为 1:1:3。此外,针刺组大鼠进一步随机分为 3 个亚组,包括内关组、尺泽组和合谷组,每组 8 只。

Preparation of AMI Model
心肌梗死模型制备

Rats were anesthetized using an intraperitoneal injection of 10 % 10 % 10%10 \% chloral hydrate ( 0.4 mL / 100 g 0.4 mL / 100 g 0.4mL//100g0.4 \mathrm{~mL} / 100 \mathrm{~g} ), and a mini rodent ventilator (Kent PhysioSuite, USA) was conducted to provide oxygen (tidal volume ratio: 1 mL / 100 g 1 mL / 100 g 1mL//100g1 \mathrm{~mL} / 100 \mathrm{~g}; inspiration and expiration ratio: 1 : 1 ) 1 : 1 ) 1:1)1: 1). The rats in the sham-operated, AMI and acupuncture groups received thoracotomy. The left anterior descending (LAD) coronary artery was permanently ligated in the AMI and acupuncture groups and left unligated in the sham-operated group. Electrocardiogram (ECG, PowerLab system, AD instruments, Australia) was monitored throughout the operation. Three needles, which were punctured in the two foot and right palm, were connected with electrodes to record the II lead of ECG, S-T segment elevated over 100 mV from the baseline was recruited as an index of myocardial ischemia. Furthermore, blood perfusion of the heart and left forelimb using LSPI technology was monitored.
大鼠通过腹腔注射 0 号氯醛水合物(1)进行麻醉,并使用小型啮齿动物呼吸机(Kent PhysioSuite,美国)提供氧气(潮气量比:2;吸呼比:3)。假手术组、AMI 组和针灸组的动物接受了开胸手术。AMI 组和针灸组永久结扎了左前降支(LAD)冠状动脉,而假手术组未结扎。手术期间全程监测心电图(ECG,PowerLab 系统,AD instruments,澳大利亚)。两足和右掌各穿刺三针,连接电极记录 ECG 的 II 导联,S-T 段基线抬高超过 100 mV 的作为心肌缺血指标。此外,使用 LSPI 技术监测心脏和左前肢的血流量。

Acupuncture Intervention
针灸干预

All rats in the acupuncture group received acupuncture intervention. The rats were anaesthetized by intraperitoneal injection of 10% chloral hydrate ( 0.4 mL / 100 g 0.4 mL / 100 g 0.4mL//100g0.4 \mathrm{~mL} / 100 \mathrm{~g} ), fixed and operated on previously described. To analyze the acupoint specificity in myocardial ischemia rats, LU 7 and LI 10 were selected as the control acupoints for PC 6, which are located on the forelimb and belong to the Lung meridian of HandTaiyin and Large Intestine meridian of Hand-Yangming, respectively, having little connection with the heart. All the acupuncture points in the study were well-positioned according to textbook of experimental acupuncture. ( 21 ) ( 21 ) ^((21)){ }^{(21)} A needle ( 0.30 mm × 25 mm 0.30 mm × 25 mm 0.30mmxx25mm0.30 \mathrm{~mm} \times 25 \mathrm{~mm}, Suzhou Medical Supplies
所有针灸组的大鼠接受了针灸干预。大鼠通过腹腔注射 10%水合氯醛( 0.4 mL / 100 g 0.4 mL / 100 g 0.4mL//100g0.4 \mathrm{~mL} / 100 \mathrm{~g} )进行麻醉,然后进行固定和手术操作,具体方法如前所述。为了分析心肌缺血大鼠的穴位特异性,选取手太阴肺经的尺泽(LU 7)和手阳明大肠经的合谷(LI 10)作为足三里(PC 6)的对照穴位,它们分别位于前肢,分别属于手太阴肺经和手阳明大肠经,与心脏的联系较少。研究中所有针灸穴位均按照实验针灸学教科书准确定位。 ( 21 ) ( 21 ) ^((21)){ }^{(21)} 使用一根针( 0.30 mm × 25 mm 0.30 mm × 25 mm 0.30mmxx25mm0.30 \mathrm{~mm} \times 25 \mathrm{~mm} ,苏州医疗器械)
Co., Ltd., China) was gently inserted in these acupoints. PC 6 was inserted perpendicularly at a depth of 1 mm , and LI 10 was similarly inserted at a depth of 5 mm . Conversely, LU 7 was inserted obliquely in the direction of shoulders at a depth of 1 mm . The needle was slowly rotated for 15 min during the acupuncture session. The rats in the other groups were anaesthetized and fixed without further intervention.
中国某有限公司生产的毫针轻轻插入这些穴位。PC6 穴垂直插入,深度为 1 毫米,LI10 穴同样插入,深度为 5 毫米。相反,LU7 穴以斜向肩部的方向插入,深度为 1 毫米。在针刺过程中,缓慢旋转针柄 15 分钟。其他组的动物被麻醉并固定,无进一步干预。

Measurement of LSPI  LSPI 的测量

The LSPI observation was conducted in the lab at a constant temperature of 30 32 C 30 32 C 30-32^(@)C30-32^{\circ} \mathrm{C} and a relative humidity of 80 % 90 % 80 % 90 % 80%-90%80 \%-90 \%. The exposed heart and left forelimb were placed approximately 11.5 ± 0.3 cm 11.5 ± 0.3 cm 11.5+-0.3cm11.5 \pm 0.3 \mathrm{~cm} below the laser scanner. The entire observation was conducted in a shielding chamber without direct sunlight, infrared radiation, or ventilation.
LSPI 观察在实验室进行,温度恒定为 30 32 C 30 32 C 30-32^(@)C30-32^{\circ} \mathrm{C} ,相对湿度为 80 % 90 % 80 % 90 % 80%-90%80 \%-90 \% 。暴露的心脏和左前肢放置在激光扫描仪下方约 11.5 ± 0.3 cm 11.5 ± 0.3 cm 11.5+-0.3cm11.5 \pm 0.3 \mathrm{~cm} 处。整个观察在屏蔽室内进行,没有阳光直射、红外辐射或通风。
A Moor-FLPI LSPI (Moor instruments Ltd., Axminster, UK) was used in this study. Scanning was run in a low density of 25 fps , the time interval was 1 s , exposure time was 20 ms , and 10 frames were continually scanned at each time point ( 10 frames were averaged into a single frame to obtain the mean HBP at each time point). The instrument system can simultaneously record the LSPI and the digital coded image of the heart and left forelimb (the actual positional image of the examined part). Subsequently, heart and pericardium meridian were chosed as the region of interest (ROI) in each group. Based on the CM theory, the pericardium meridian runs in the middle of the inner side of the upper arm. Therefore, the middle region of the inner side of the upper arm was chose as the monitored area of pericardium meridian perfusion (PMP). Meanwhile, the whole heart of rats was monitored and considered as the heart microcirculation perfusion (HMP). The perfusion unit (PU) of the ROI was calculated and analyzed by the PSI System (Figure 1).
本研究使用了 Moor-FLPI LSPI(Moor instruments Ltd., Axminster, UK)设备。扫描以 25 fps 的低密度进行,时间间隔为 1 秒,曝光时间为 20 毫秒,每个时间点连续扫描 10 帧(将 10 帧平均为 1 帧以获得每个时间点的平均 HBP)。该仪器系统可同时记录 LSPI 以及心脏和左前肢的数字编码图像(即检查部位的实际位置图像)。随后,在每个组中将心脏和心包经选为感兴趣区域(ROI)。根据 CM 理论,心包经位于上臂内侧中部。因此,选择上臂内侧中部作为心包经灌注(PMP)的监测区域。同时,监测大鼠整个心脏并视为心脏微循环灌注(HMP)。通过 PSI 系统计算并分析 ROI 的灌注单位(PU)(图 1)。
The rats in each group were bound and operated, and ROI was marked and exposed to air before sampling to adapt to the environment. The rats were anesthetized in a supine position. Fifteen minutes later, the blood perfusion data of the marked spot was recorded. For rats in the acupuncture groups, the blood perfusion images were recorded before, during and after acupuncture for 15 min , respectively. In contrast, the sham-operated and AMI groups were continuously recorded for 45 min . The blood perfusion images were saved and analyzed
每组大鼠被绑扎并实施手术,采样前标记区域暴露于空气中以适应环境。大鼠采取仰卧位进行麻醉。十五分钟后,记录标记区域的血流量数据。对于针灸组大鼠,分别记录针灸前、中、后十五分钟的血流量图像。相比之下,假手术组和 AMI 组持续记录四十五分钟。血流量图像被保存并进行分析

Figure 1. Diagram for Data Acquisition
图 1. 数据采集示意图

Notes: 1: heart microcirculation perfusion; 2: pericaridium meridian microcirculation perfusion
注:1:心脏微循环灌注;2:心包经微循环灌注

by the image review program of Moor-FLPI V2.0 software. The blood perfusion levels of 3 groups were compared at 3 time points.
由 Moor-FLPI V2.0 软件的图像审查程序进行分析。三组的血流量水平在三个时间点进行比较。

Statistical Analysis  统计分析

SPSS 21.0 software was used for statistical analysis. The value of blood perfusion at each time point was expressed as mean ± ± +-\pm standard deviation ( x ¯ ± s ) ( x ¯ ± s ) ( bar(x)+-s)(\bar{x} \pm s). Different values of blood perfusion between the time points were analyzed by repeated measurement of analysis of variance (ANOVA) and the statistical method of pairwise comparison between groups was the least significant difference (LSD). P < 0.05 P < 0.05 P < 0.05P<0.05 was considered as having statistical differences.
统计分析采用 SPSS 21.0 软件进行。每个时间点的血流量值以均值±标准差表示。不同时间点的血流量值差异采用重复测量方差分析(ANOVA)进行检验,组间两两比较采用最小显著差异(LSD)检验法。P<0.05 被认为具有统计学意义。

RESULTS  结果

Baseline Data in Different Groups
不同组别基线数据

The baseline weight and heart rate of rats showed no significant differences ( P > 0.05 P > 0.05 P > 0.05P>0.05, Table 1 ).
大鼠的基线体重和心率无显著差异( P > 0.05 P > 0.05 P > 0.05P>0.05 ,表 1)。
Table 1. Comparison of Baseline Weight and Heart Rate in Each Group ( x ± s x ¯ ± s bar(x)+-s\overline{\mathbf{x}} \pm \mathbf{s} )
表 1. 各组基线体重和心率的比较( x ± s x ¯ ± s bar(x)+-s\overline{\mathbf{x}} \pm \mathbf{s}
Group  组别 n n nn Weight (g)  体重(g) Heart rate (time/min)  心率(时间/分钟)
Sham-operated  假手术组 8 233.63 ± 16.18 233.63 ± 16.18 233.63+-16.18233.63 \pm 16.18 259.75 ± 64.28 259.75 ± 64.28 259.75+-64.28259.75 \pm 64.28
AMI 8 237.88 ± 24.69 237.88 ± 24.69 237.88+-24.69237.88 \pm 24.69 262.42 ± 39.63 262.42 ± 39.63 262.42+-39.63262.42 \pm 39.63
PC 6 8 243.86 ± 34.17 243.86 ± 34.17 243.86+-34.17243.86 \pm 34.17 315.79 ± 46.25 315.79 ± 46.25 315.79+-46.25315.79 \pm 46.25
LU 7 8 222.63 ± 8.45 222.63 ± 8.45 222.63+-8.45222.63 \pm 8.45 333.71 ± 49.63 333.71 ± 49.63 333.71+-49.63333.71 \pm 49.63
LI 10 8 231.57 ± 10.69 231.57 ± 10.69 231.57+-10.69231.57 \pm 10.69 353.33 ± 36.08 353.33 ± 36.08 353.33+-36.08353.33 \pm 36.08
Group n Weight (g) Heart rate (time/min) Sham-operated 8 233.63+-16.18 259.75+-64.28 AMI 8 237.88+-24.69 262.42+-39.63 PC 6 8 243.86+-34.17 315.79+-46.25 LU 7 8 222.63+-8.45 333.71+-49.63 LI 10 8 231.57+-10.69 353.33+-36.08| Group | $n$ | Weight (g) | Heart rate (time/min) | | :--- | :--- | :--- | :--- | | Sham-operated | 8 | $233.63 \pm 16.18$ | $259.75 \pm 64.28$ | | AMI | 8 | $237.88 \pm 24.69$ | $262.42 \pm 39.63$ | | PC 6 | 8 | $243.86 \pm 34.17$ | $315.79 \pm 46.25$ | | LU 7 | 8 | $222.63 \pm 8.45$ | $333.71 \pm 49.63$ | | LI 10 | 8 | $231.57 \pm 10.69$ | $353.33 \pm 36.08$ |

ECG Changes in Different Groups
不同组别的心电图变化

S-T segments were noticeably elevated after ligation of LAD, indicating the model replication was successful. Acupuncture of PC 6 obviously improved the abnormality of ECG, and the effects were better than the LU 7 and LI 10 groups according to ECG record (Figure 2).
结扎 LAD 后 S-T 段明显升高,表明模型复制成功。针刺 PC 6 明显改善了心电图异常,根据心电图记录,其效果优于 LU 7 和 LI 10 组(图 2)。

  1. ©The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021
    ©《中国中西医结合杂志》出版社和 Springer-Verlag GmbH 德国,Springer Nature 2021 年部分

    *Supported by the Project Acupoint Sensitization Research of the National Natural Science Foundation of China (No. 81590951), National Basic Research Program of China (No. 2012CB518501), and the National Natural Science Foundation of China (No. 81373559)
    *由国家自然科学基金项目(编号:81590951)、国家基础研究计划(编号:2012CB518501)和国家自然科学基金项目(编号:81373559)资助
    1. College of Acupuncture, Moxibustion and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu (610075), China; 2. College of Acupuncture, Moxibustion and Tuina, Nanjing University of Chinese Medicine, Nanjing (210023), China; 3. Department of Acupuncture and Moxibustion, Xin Du Hospital of Traditional Chinese Medicine, Chengdu (610599), China; 4. The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, Guangdong Province (518005), China
      成都中医药大学针灸推拿学院,成都(610075),中国;2. 南京中医药大学针灸推拿学院,南京(210023),中国;3. 成都中医药大学新都医院针灸科,成都(610599),中国;4. 广州中医药大学第四临床医学院,广东省深圳市(518005),中国

      DOI: https://doi.org/10.1007/s11655-021-3294-9