In this study, cultivated and wild Chinese cherry, and sweet cherry accessions were collected from Pujiang, Yingjing Sichuan and Zibo Shandong, respectively. The seeds were used for next experiment after excluding fruit flesh 本研究分别从浦江、荥泾四川和山东淄博收集了栽培和野生樱桃和甜樱桃种质。剔除果肉后,将种子用于下一个实验
We set three gradient gibberellin(GA3) concentration of 0, 50, 100 and 150mg//L150 \mathrm{mg} / \mathrm{L}. Fifty seeds were immersed in gibberellin(GA3) solution for 24 h in each treatment with three complicates per treatment. And then the seeds were put on the culture dish at 25^(@)C25^{\circ} \mathrm{C} to germinate. After several days, we summarized the date for germination and the number of germinated seeds. The germinating seed is formed when the length of the seed coat is half the length of the seed. Germination index and rate wert calculated by (the number of germinated seeds within the allotted period / total number of seeds) xx100%\times 100 \%, and total number of germinated seeds /// total number of seeds) xx100%\times 100 \%. 我们设置了 0、50、100 和 150mg//L150 \mathrm{mg} / \mathrm{L} 的三个梯度赤霉素 (GA3) 浓度。在每个处理中,将 50 颗种子浸泡在赤霉素 (GA3) 溶液中 24 h,每个处理 3 个复杂剂。然后将种子放在培养皿上 25^(@)C25^{\circ} \mathrm{C} 发芽。几天后,我们总结了发芽日期和发芽种子的数量。当种皮的长度是种子长度的一半时,就会形成发芽的种子。发芽指数和发芽率的计算公式为(分配期内发芽的种子数 / 种子总数), xx100%\times 100 \% 以及发芽种子 ///xx100%\times 100 \% 总数 。
Significant differences between the means of the treatments were determined with 95% confidence ( pp < 0.05) limit by Duncan multiple range test using SPSS18.0 (IBM, USA). Data are shown as the means of three replicates. 通过使用 SPSS18.0 (IBM, USA) 的 Duncan 多范围检验,以 95% 置信度 ( pp < 0.05) 限确定处理均值之间的显著差异。数据显示为 3 个仿行的均值。