Calculate the number of CFU of presumptive bifidobacteria per gram of product, , using the equation
计算每克产品中推定的双歧杆菌的 CFU 数， 使用以下公式

where

is the sum of colonies counted on all dishes retained (8.8);
是所有保留的培养皿上计算的菌落总和 （8.8）;

is the number of dishes retained in the first countable dilution;
是第一可数稀释液中保留的培养皿数量;

is the number of dishes retained in the second dilution;
是第二次稀释中保留的培养皿数量;

is the number of dishes retained in the third dilution;
是第三次稀释中保留的培养皿数量;

is the dilution factor corresponding to the first countable dilution retained.
是对应于保留的第一个可计数稀释的稀释因子。

If there are only two countable dilutions, modify the equation to
如果只有两个可计数的稀释度，则将公式修改为

Determine the reliability of the colony counts obtained from parallel plates and subsequent dilution steps according to ISO 14461-2||DF 169-2. For the calcularion of the result, use only reliable counts (see also ISO 7218).
根据 ISO 14461-2 确定从平行板获得的菌落计数和后续稀释步骤的可靠性||DF 169-2型。对于结果的计算，仅使用可靠的计数（另请参阅 ISO 7218）。

Express the results to two significant figures as the number of CFU of bifidobacteria per gram of product, representing a number between 1,0 and 9,0 multiplied by the appropriate power of 10 . For the validity of results see 10.4 .
将结果表示为两个有效数字，即每克产品的双歧杆菌 CFU 数，表示 1,0 和 9,0 之间的数字乘以 10 的适当幂。有关结果的有效性，请参见 10.4 。

If the last figure is below 5, the precedjing figure is not modified. If the last figure is 5 or more, increase the preceding figure by one unit. Proceed stepwise until two significant figures are obtained (see ISO 7218).
如果最后一个数字小于 5，则不会修改前面的数字。如果最后一个数字是 5 或更多，则将前一个数字增加一个单位。逐步进行，直到获得两个有效数字（参见 ISO 7218）。

EXAMPLE 1 Assuming that a coun of bifidobacteria on the medium gave the results shown below (two Petri dishes per dilution incubated), the final result can be calculaled as shown.
实施例 1 假设培养基上的一组双歧杆菌得到如下所示的结果（每次孵育稀释液两个培养皿），最终结果可以如图所示计算。

Table 1-Example 1 表1-示例1

Insertion of the figures into Equalion (1) gives
将数字插入均衡 （1） 可得到

EXAMPLE 2 Assuming that a count of bifidobacteria on the medium gave the results shown below (two Petri dishes per dilution incubated), the final resull can be calculated as shown.
实施例 2 假设培养基上的双歧杆菌计数得到如下所示的结果（每个稀释液孵育两个培养皿），则最终回收量可以如图所示计算。

Table 2 - Example 2
表 2 - 示例 2

Insertion of the figures into Equation (1) gives
将数字代入公式（1）中可得到

Details of an interlaboratory test done in 2006 on the precision of the method are summarized in Annex A (see also References [12][13]). The repeatability and the reproducibility limits were determined by using a powdered probiotic infant milk formula as well as six different probiotic yogurt products containing various bifidobacterial strains commercially used both in Europe and Japan.
2006年对该方法的精确度进行的实验室间测试的细节总结在附件A中（另见参考文献[12][13]）。重复性和再现性限值通过使用含有益生菌婴儿配方奶粉以及含有欧洲和日本商业使用的各种双歧杆菌菌株的六种不同的益生菌酸奶产品来确定。

The values derived from the interlaboratory test may not be applicable to concentration ranges and matrices other than those given. The tested concentration ranges of the bifidobacterial strains within the selected products are representative for the worldwide market and in accordance with Codex Stan 243:2003 [6].
从实验室间测试得出的值可能不适用于给定范围和基质以外的浓度范围和基质。所选产品中双歧杆菌菌株的测试浓度范围具有全球市场代表性，并符合 Codex Stan 243：2003 [6]。

The repeatability is the closeness of agreement between successive and independent results obtained by the same method on identical test material. under the same conditions (apparatus, operator, laboratory and short intervals of time); i.e. repeatability conditions.
重复性是用相同方法在相同测试材料上获得的连续和独立结果之间的一致性。在相同的条件下（仪器、操作员、实验室和短时间间隔）;即可重复性条件。

The repeatability limit, , is the value less than or equal to which the absolute difference between two test results (presumptive bifidobacteria per gram, converted to logarithms to the base 10) obtained under repeatability conditions is expedted to lie with a probability of (see ISO 3534-1 [2], ISO 5725-1 [3], ISO 5725-2 [4] and ISO ).
重复性限值 ，是在重复性条件下获得的两个测试结果（推定的每克双歧杆菌，转换为以 10 为底的对数）之间的绝对差值小于或等于该值的值，概率为 （参见 ISO 3534-1 [2]、ISO 5725-1 [3]、ISO 5725-2 [4] 和 ISO ）。

Table 3, second column from the right, shows the repeatability of different milk products obtained in the ring trial in 2006, expressed as repeatability limit, . These results were calculated by applying the robust analyses in ISO 16140[5] taking into decount all variations (deviations) reflecting usual and practical conditions.
表3右起第二列，显示了2006年环形试验中获得的不同奶制品的重复性，表示为重复性极限， 。这些结果是通过应用ISO 16140[5]中的稳健分析计算得出的，并考虑了反映通常和实际条件的所有变化（偏差）。

The reproducibility is the closeness of agreement between single test results on identical test material using the same method and obtained by operators in different laboratories using different equipment; i.e. reproducibility conditfons.
再现性是指使用相同方法对相同测试材料进行的单个测试结果与不同实验室的操作人员使用不同设备获得的一致性的接近程度;即可重复性条件。

The reproducibility limit, , is the value less than or equal to which the absolute difference between two test results (presumptive bifidobacteria CFU per gram, converted to logarithms to the base 10) obtained under reproducibility conditions is expected to lie with a probability of (see ISO 3534-1 [2], ISO 5725-1 [3], ISO 5725-2 [4] and ISO .
再现性极限 是小于或等于在再现性条件下获得的两个测试结果（假定的双歧杆菌 CFU/克，转换为以 10 为基数的对数）之间的绝对差异小于或等于的值，预计概率为 （参见 ISO 3534-1 [2]、ISO 5725-1 [3]、ISO 5725-2 [4] 和 ISO 。

Table 3, rightmost column, shows the reproducibility of different milk products obtaired in the ring trial in 2006 , expressed as reproducibility limit, . These results were calculated by applying the robust analyses in ISO 16140[5] taking into account all variations (deviations) reflecting usual and practical conditions.
表3，最右边一列，显示了2006年环形试验中淘汰的不同奶制品的再现性，以再现限表示 。这些结果是通过应用ISO 16140[5]中的稳健分析计算得出的，同时考虑了反映通常和实际条件的所有变化（偏差）。

Table 3 - Repeatability limits, robust, and reproducibinty limits, robust
表3 - 稳健的重复性限值和 稳健的再现性限值

Based on the similarities of the results obtained for some of the products, the precision data of the method were defined, thereby distinguisping between three different types of products (see Table 4).
基于部分产品结果的相似性，定义了该方法的精密度数据，从而区分了三种不同类型的产品（见表4）。

The results obtained for yogurt 5 exhibited irregularities possibly due to inhomogeneity and were excluded from the calculation (see References [12][13] for more details).
酸奶 5 获得的结果表现出不规则性，可能是由于不均匀性造成的，因此被排除在计算之外（更多详细信息见参考文献 [12][13]）。

Depending on the type of product, apply the following precision data (repeatability limits as well as reproducibility limits, Table 4)
根据产品类型，应用以下精度数据（重复性限值和再现性限值，表4）

a) liquid yogurts, including all yogurt drinks or similar;

b) firm and creaphy yogurts (including set-style yogurts);
b） 硬质酸奶和奶油酸奶（包括套餐式酸奶）;

c) powder prgducts, including infant milk formulae or similar.
c） 奶粉产品，包括婴儿配方奶粉或类似产品。